Coding
Part:BBa_K1998002:Design
Designed by: Shauna Winchester Group: iGEM16_Macquarie_Australia (2016-10-12)
psbTB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 573
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Genes were codon optimised for bacteria using optimiser tool at http://genomes.urv.es/ OPTIMIZER/ Restriction sites were identified using Gene Designer, and removed by codon swapping, maintaining AA sequence.
Source
The genes were sourced from Chlamydomonas rienhardtii and synthesised