Coding

Part:BBa_K1998002:Design

Designed by: Shauna Winchester   Group: iGEM16_Macquarie_Australia   (2016-10-12)


psbTB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 573
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Genes were codon optimised for bacteria using optimiser tool at http://genomes.urv.es/ OPTIMIZER/ Restriction sites were identified using Gene Designer, and removed by codon swapping, maintaining AA sequence.



Source

The genes were sourced from Chlamydomonas rienhardtii and synthesised

References